Tracking Single Secretory Granules in Live Chromaffin Cells by Evanescent-Field Fluorescence Microscopy J. A. Steyer and W. Almers Max-Planck-Institut fu¨r Medizinische Forschung, 69120 Heidelberg, Germany
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اقرأ أكثرSee also. Indole-3-acetic acid and auxin herbicides up-regulate 9-cis-epoxycarotenoid... Indole-3-acetic acid and auxin herbicides up-regulate 9-cis-epoxycarotenoid dioxygenase gene expression and abscisic acid accumulation in cleavers (Galium aparine): interaction with ethylene
اقرأ أكثر(Bachhofer, Reutlingen, Germany). Collagen was pre-pared as previously described [26]. Each entorhinal slice was embedded in a drop of collagen matrix on glass slides. The recombinant factors (neurotrophins and IL-1b) were mixed into the sterile cultivation medium con-taining MEM, 25% HBSS, 25% heat-inactivated normal
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اقرأ أكثرwasplacedindialysischambers(Dianorm,Bachhofer, Reutlingen, FRG)separatedbydialysismembranes, with a molecular cut-off of 5000 daltons (Visking, Serva, Heidelberg, FRG)anddialysed against 1 ml phosphate buffered saline (0.15 M NaCl, 0.01 M K2 HPO4, 0.05 MNaH2PO4) at pH 7.4. Dialysis was carried out at 37°C. Under these conditions equili ...
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اقرأ أكثرa Polytron homogenizer (Bachhofer, Reutlingen, Germany). All further steps were performed at 0-4°C. The homogenate was filtered through gauze and centrifuged at 500 xg for 10 min in a refrigerated centrifuge to remove debris and fibrillar material. This step was necessary to reduce varia-
اقرأ أكثرHandheld Grinding Machine Germany. Ancient German Magnetic Hand Maize Grinding Machine. Early pressure probe results have failed to corroborate the pressure bomb Balling The iron plate was also used as a magnetic stand for fixing a metal frame Tips were polished with a grinding machine Bachhofer Reutlingen Germany On the other hand Ri included a small portion of the stem resistance below the ...
اقرأ أكثرRESEARCH Open Access Interleukin-1 beta and neurotrophin-3 synergistically promote neurite growth in vitro Franc Boato1,2†, Daniel Hechler3†, Karen Rosenberger3, Doreen Lüdecke3, Eva M Peters4,5, Robert Nitsch6 and Sven Hendrix1* Abstract Pro-inflammatory cytokines such as interleukin-1 beta (IL-1b) are considered to exert detrimental effects during
اقرأ أكثرDepartment of Environmental Microbiology 1 and Division of Structural Biology, 2 GBF—German Research Centre for Biotechnology, Mascheroder Weg 1, D-38124 Braunschweig, Germany Received 9 ...
اقرأ أكثرPlant Physiology, December 1999, Vol. 121, pp. 1191-1205, ?D 1999 American Society of Plant Physiologists Direct Measurement of Xylem Pressure in Leaves of Intact
اقرأ أكثرon Petriperm dishes (Bachhofer, Reutlingen, Germany), which show less UV absorption than plastic petri dishes. To investigate the time course of Cl- currents activated by synaptic release of 5-HT, Retzius P-cell synapses were grown in culture. We predominantly analyzed Retzius …
اقرأ أكثرAll solvents were removed in a vacuum centrifuge (Bachhofer, Reutlingen, Germany). The Separation was carried out on a semi-preparative column (500 mm X 8 mm), fllled with Lichrospher Diol 5 μηι 100 (Merck, Darmstadt). This column was protected by a pre-column (20 mm x …
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اقرأ أكثرTo determine the EGFP‐fluorescence as a marker for the extent of virus replication, 5 × 10 5 cells, coincubated with NDFL‐EGFP as described, were washed twice with PBS, transferred to steril microscope slides by centrifugation (500 U/min, 3 min) using a Cytospin device (Bachhofer, Reutlingen, Germany) and then analyzed using a confocal ...
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اقرأ أكثرPer sample protein solution corresponding to 5 μg of total protein was dried using a vacuum concentrator (Bachhofer, Reutlingen, Germany). Protein lyophilisates were redissolved in 1 M Urea/50 mM NH4HCO3 and sonicated for 10 min (Bandelin, Berlin, Germany). Cysteine residues were reduced for 30 min in 45 mM dithiothreitol/50 mM NH4HCO3 at room ...
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اقرأ أكثرBriefly, ginger rhizomes were cut into small dices after peeling, and then frozen at −20 °C. The ginger dices were homogenized in 5 volumes (w/v) of chilled acetone by a Polytron homogenizer D-9 (Bachhofer, Reutlingen, Germany). The homogenate was filtered, and the residue was rinsed with 5 volumes of chilled acetone.
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اقرأ أكثرAbstract. A modified genomic DNA subtraction hybridization approach for the design of strain specific PCR systems was developed and evaluated. The method is ba
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